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Original Research 


Hexane fraction of Costus afer Ker Gawl leaves inhibited mitochondrial membrane permeability transition, F1F0 ATPase and scavenged nitric oxide and hydrogen peroxide

Godswill Nduka Anyasor, Funmilayo D Onajobi, Odutola Osilesi, Olugbenga Adebawo.

Abstract
Costus afer ker Gawl. is an indigenous medicinal plant used as therapy in the treatment of inflammatory disease such as rheumatoid arthritis. This study was designed to investigate the chemoprotective potentials of the hexane fraction of C. afer leaves (CAHFL) on some biochemical biomarkers such as mitochondrial membrane permeability transition (MPT), F1F0 ATPase, nitric oxide (NOĽ) and hydrogen peroxide (H2O2) activities using spectrophotometric methods. Results showed that isolated mitochondria fractions from normal rats preloaded with 10 - 60 ╬╝g/ml CAHFL significantly (P<0.05) inhibited Ca2+-induced MPT by 85.66 - 90.47% respectively in vitro. Also, in vivo study showed that mitochondria fractions isolated from formaldehyde-induced arthritic rats orally treated with 50 - 250 mg/kg body weight CAHFL for 7 days significantly (P<0.05) inhibited mitochondrial MPT by 52.22 - 65.0% respectively. Furthermore, there was a significant reduction (P<0.05) in mitochondrial F1F0 ATPase activity (3.93 ▒ 0.01 mg/ml Pi) at pH 7.4 by 10 ╬╝g/ml CAHLF compared with F1F0 ATPase activity (6.60 ▒ 0.03 mg/ml Pi) by 10 ╬╝g/ml 2,4-dinitrophenol. In another study, varied concentrations of CAHFL scavenged NOĽ and H2O2 in a concentration dependent manner with an IC50 of 1.77 mg/ml and 0.33 mg/ml respectively. These data indicated that CAHFL possesses the capacity to inhibit mitochondrial MPT, F1F0 ATPase, nitric oxide and hydrogen peroxide activities. This could further explain the medicinal potentials of CAHLF, thereby indicating that CAHLF could be a choice candidate in the drug discovery process.

Key words: Costus afer; mitochondria; ATPase; nitric oxide; hydrogen peroxide


 
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How to Cite this Article
Pubmed Style

Godswill Nduka Anyasor, Funmilayo D Onajobi, Odutola Osilesi, Olugbenga Adebawo. Hexane fraction of Costus afer Ker Gawl leaves inhibited mitochondrial membrane permeability transition, F1F0 ATPase and scavenged nitric oxide and hydrogen peroxide. A J Physiol Biochem Pharmacol. 2014; 3(2): 79-85. doi:10.5455/jib.20140723062605


Web Style

Godswill Nduka Anyasor, Funmilayo D Onajobi, Odutola Osilesi, Olugbenga Adebawo. Hexane fraction of Costus afer Ker Gawl leaves inhibited mitochondrial membrane permeability transition, F1F0 ATPase and scavenged nitric oxide and hydrogen peroxide. http://www.ajpbp.com/?mno=151913 [Access: November 18, 2018]. doi:10.5455/jib.20140723062605


AMA (American Medical Association) Style

Godswill Nduka Anyasor, Funmilayo D Onajobi, Odutola Osilesi, Olugbenga Adebawo. Hexane fraction of Costus afer Ker Gawl leaves inhibited mitochondrial membrane permeability transition, F1F0 ATPase and scavenged nitric oxide and hydrogen peroxide. A J Physiol Biochem Pharmacol. 2014; 3(2): 79-85. doi:10.5455/jib.20140723062605



Vancouver/ICMJE Style

Godswill Nduka Anyasor, Funmilayo D Onajobi, Odutola Osilesi, Olugbenga Adebawo. Hexane fraction of Costus afer Ker Gawl leaves inhibited mitochondrial membrane permeability transition, F1F0 ATPase and scavenged nitric oxide and hydrogen peroxide. A J Physiol Biochem Pharmacol. (2014), [cited November 18, 2018]; 3(2): 79-85. doi:10.5455/jib.20140723062605



Harvard Style

Godswill Nduka Anyasor, Funmilayo D Onajobi, Odutola Osilesi, Olugbenga Adebawo (2014) Hexane fraction of Costus afer Ker Gawl leaves inhibited mitochondrial membrane permeability transition, F1F0 ATPase and scavenged nitric oxide and hydrogen peroxide. A J Physiol Biochem Pharmacol, 3 (2), 79-85. doi:10.5455/jib.20140723062605



Turabian Style

Godswill Nduka Anyasor, Funmilayo D Onajobi, Odutola Osilesi, Olugbenga Adebawo. 2014. Hexane fraction of Costus afer Ker Gawl leaves inhibited mitochondrial membrane permeability transition, F1F0 ATPase and scavenged nitric oxide and hydrogen peroxide. American Journal of Physiology, Biochemistry and Pharmacology, 3 (2), 79-85. doi:10.5455/jib.20140723062605



Chicago Style

Godswill Nduka Anyasor, Funmilayo D Onajobi, Odutola Osilesi, Olugbenga Adebawo. "Hexane fraction of Costus afer Ker Gawl leaves inhibited mitochondrial membrane permeability transition, F1F0 ATPase and scavenged nitric oxide and hydrogen peroxide." American Journal of Physiology, Biochemistry and Pharmacology 3 (2014), 79-85. doi:10.5455/jib.20140723062605



MLA (The Modern Language Association) Style

Godswill Nduka Anyasor, Funmilayo D Onajobi, Odutola Osilesi, Olugbenga Adebawo. "Hexane fraction of Costus afer Ker Gawl leaves inhibited mitochondrial membrane permeability transition, F1F0 ATPase and scavenged nitric oxide and hydrogen peroxide." American Journal of Physiology, Biochemistry and Pharmacology 3.2 (2014), 79-85. Print. doi:10.5455/jib.20140723062605



APA (American Psychological Association) Style

Godswill Nduka Anyasor, Funmilayo D Onajobi, Odutola Osilesi, Olugbenga Adebawo (2014) Hexane fraction of Costus afer Ker Gawl leaves inhibited mitochondrial membrane permeability transition, F1F0 ATPase and scavenged nitric oxide and hydrogen peroxide. American Journal of Physiology, Biochemistry and Pharmacology, 3 (2), 79-85. doi:10.5455/jib.20140723062605





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