E-ISSN 2578-7322
 

Original Research 


Analysis of Collagen Type II and X in Ageing and Osteoarthritic Articular Cartilage

Neeru Goyal, Madhur Gupta, Kiran deep Gill.

Cited by (1)

Abstract
Objective: Collagens identified in articular cartilage include the cartilage specific collagen types II, IX, X and XI as well as the more universally distributed types III, V, VI, XII and XIV. Type II collagen is the predominant and characteristic collagen of the articular cartilage, composed of a triple helix of three identical α chains. The hypertrophic chondrocytes of calcified zone of articular cartilage synthesize type X collagen in addition to the type II collagen. The present study was conducted to observe the changes in the collagenase activity in osteoarthritis by detecting TCA fragment of the α chain of collagen type II. The expression of native collagen type X was also observed in the osteoarthritic articular cartilage by SDS-PAGE and western blotting. Methods: 22 osteoarthritic cartilage specimens were obtained from patients with primary osteoarthritis (46-81years) undergoing total knee replacement. 12 age-matched (41-86years) and 14 young (16-40years) non-osteoarthritic control cartilage specimens were obtained from the cadavers in the department of Anatomy and from patients undergoing lower limb amputation in Trauma center of PGIMER, Chandigarh. The cartilage specimens were processed for SDS-PAGE and western blotting. Results: Analysis of the α-chymotrypsin extracts of the articular cartilage for TCA fragment (75kDa) of collagen type II and native collagen type X (60kDa) revealed a much higher content in the osteoarthritic group. Conclusions: The features of the articular cartilage from the osteoarthritic group were markedly different from the non-osteoarthritic age-matched articular cartilage suggesting that OA is not an inevitable feature of aging.

Key words: cartilage, collagen, osteoarthritis, western blotting, ageing


 
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How to Cite this Article
Pubmed Style

Neeru Goyal, Madhur Gupta, Kiran deep Gill. Analysis of Collagen Type II and X in Ageing and Osteoarthritic Articular Cartilage. A J Physiol Biochem Pharmacol. 2012; 1(1): 12-16. doi:10.5455/jib.1333344473


Web Style

Neeru Goyal, Madhur Gupta, Kiran deep Gill. Analysis of Collagen Type II and X in Ageing and Osteoarthritic Articular Cartilage. http://www.ajpbp.com/?mno=18291 [Access: November 18, 2018]. doi:10.5455/jib.1333344473


AMA (American Medical Association) Style

Neeru Goyal, Madhur Gupta, Kiran deep Gill. Analysis of Collagen Type II and X in Ageing and Osteoarthritic Articular Cartilage. A J Physiol Biochem Pharmacol. 2012; 1(1): 12-16. doi:10.5455/jib.1333344473



Vancouver/ICMJE Style

Neeru Goyal, Madhur Gupta, Kiran deep Gill. Analysis of Collagen Type II and X in Ageing and Osteoarthritic Articular Cartilage. A J Physiol Biochem Pharmacol. (2012), [cited November 18, 2018]; 1(1): 12-16. doi:10.5455/jib.1333344473



Harvard Style

Neeru Goyal, Madhur Gupta, Kiran deep Gill (2012) Analysis of Collagen Type II and X in Ageing and Osteoarthritic Articular Cartilage. A J Physiol Biochem Pharmacol, 1 (1), 12-16. doi:10.5455/jib.1333344473



Turabian Style

Neeru Goyal, Madhur Gupta, Kiran deep Gill. 2012. Analysis of Collagen Type II and X in Ageing and Osteoarthritic Articular Cartilage. American Journal of Physiology, Biochemistry and Pharmacology, 1 (1), 12-16. doi:10.5455/jib.1333344473



Chicago Style

Neeru Goyal, Madhur Gupta, Kiran deep Gill. "Analysis of Collagen Type II and X in Ageing and Osteoarthritic Articular Cartilage." American Journal of Physiology, Biochemistry and Pharmacology 1 (2012), 12-16. doi:10.5455/jib.1333344473



MLA (The Modern Language Association) Style

Neeru Goyal, Madhur Gupta, Kiran deep Gill. "Analysis of Collagen Type II and X in Ageing and Osteoarthritic Articular Cartilage." American Journal of Physiology, Biochemistry and Pharmacology 1.1 (2012), 12-16. Print. doi:10.5455/jib.1333344473



APA (American Psychological Association) Style

Neeru Goyal, Madhur Gupta, Kiran deep Gill (2012) Analysis of Collagen Type II and X in Ageing and Osteoarthritic Articular Cartilage. American Journal of Physiology, Biochemistry and Pharmacology, 1 (1), 12-16. doi:10.5455/jib.1333344473





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