E-ISSN 2578-7322
 

Original Research 


The role of urinary 5- hydroxyindoleacetic acid determination in diagnosis of acute appendicitis

Hamid Hindi Sarhan, Aseel Fawzi Hatroosh, Amina Hamed Alobaidi.

Cited by (3)

Abstract
Abstract
Background. Appendicitis is associated with high rate of wrong diagnosis.
Objective. To evaluate the predictive value of 5-HIAA in diagnosis of appendicitis.
Methods. Spot urine samples were collected from 147 patients with appendicitis and 45 healthy controls. 5-HIAA was detected in urine samples in all patients using ELISA method.
Results. The mean 5-HIAA level was 4.5 µmol/mmol creatinine in healthy individuals, 5.8 µmol/mmol creatinine in patients with wrongly diagnosed appendicitis, and 15.7 µmol/mmol creatinine in patients with appendicitis. The difference between patients group with appendicitis and other 2 groups was statistically highly significant (P<0.001). However, the difference between the normal individuals group and wrongly diagnosed group was statistically non significant. The test had 81% sensitivity and 85% specificity.
Conclusion. This study indicated that determination of 5-HIAA as non- invasive test was with predictive value in diagnosis of appendicitis. Thus reducing the malpractice in surgical operation in Iraqi community.

Key words: Keywords: 5-HIAA, acute appendicitis, diagnosis.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Hamid Hindi Sarhan
Articles by Aseel Fawzi Hatroosh
Articles by Amina Hamed Alobaidi
on Google
on Google Scholar
Article Statistics
 Viewed: 2598
Downloaded: 724
Cited: 3

REFERENCES
1. Luigi Santacroce, Juan B Ochoa. Appendicitis. Medscopes continually updated clinical reference Web MD. 2009 may; Available from URL: http://emedicine.medscape.com/article/195778-overview
2. Elezary R, Maly A, Khalaileh A, Rubinstein C, Olstain-Pops K, Almogy G, Rivkind AI, Mintz Y. Schistosomiasis and acute appendicitis. IMAJ. 2005;7:533-4.
3. Korner H, Sondenaa K, Soreide J A, Anderson E, Nysted A. Incidance of acute non-perforated and perforated appendicitis :age specific and sex specific analysis. World J Sur. 1997;21:313-7. [DOI via Crossref]   
4. Jerry L. O, Reginald W. D, Wendell Yap, Jared D. Imaging for Suspected Appendicitis. Am Fam Phys 2005;71(1):71-8.
5. Mihmanli M, Uysalol M, Coskun, H, Demir U, Dilege E, Eroglu T. The value of 5-hydroxyindolacetic acid levels in spot urine in the diagnosis of acute appendicitis. Ulus Travma Derg 2004;10(3):173-6.
6. Bolandparvaz S, Vasei M, Owji AA, Ata-Ee N, Amin A, Daneshbod Y, Hosseini SV. Urinary 5-hydroxy indole acetic acid as a test for early diagnosis of acute appendicitis. Clin Biochem 2004;37(11):985-9. [DOI via Crossref]    [Pubmed]   
7. Nampet N, Tanthangtham A, Sakonya D. Acute Appendicitis: A 5-year Review of Histopathology and Clinical Presentation. The Thai Journal of Surgery 2003;24:81-4.
8. Mowlavi GH, Massoud J, Mobedi I, Rezaian M, Solaymani S Mohammad, Mostoufi NE, et al. Enterobius vermicularis: A Controversial Cause of Appendicitis. Iranian J Publ Health 2004;33(3):27-31.
9. Ramezani MA, Dehghani MR. Relationship between Enterobius vermicularis and the incidence of acute appendicitis. Southeast Asian J Trop Med Public 2007;38(1):20-3.
10. AL-Salami Faiz K. Bacteriological Study Regarding Acute Appendicitis. M. Sc. Thesis, Coll. Med., Univ. Babylon. Jun 2006.
11. Cerva L, Schrottenbaum M, Kliment V. Intestinal parasites: a study of human appendices. Folia Parasitol (Praha). 1984;38:5-9.
12. Bernard M, Jaffe and David H Berger. The Appendix. In: Brunicardi F C, Andersen DK, Billiar TR, Dunn DL, Hunter JG, editors. Schwartz&#1615; s Principles of Surgery. 8th ed. New York: McGraw-Hill Health Pub Division; 2005. p. 1119-34.
13. Addiss DG, Shaffer N, Fowler BS, Tauxe RV. The epidemiology of appendicitis and appendectomy in the United States. Am J Epidemiol 1990;132:910. [Pubmed]   
14. Nisar Ali, Muhammad Sadiq, Jehangir Bacha, Ghulam Hadi. Correlation of clinical and histological diagnosis of acute Appendicitis. J Postgrand Med Inst. 2003;17(2):254-7.
15. Colleran G, Heneghan H, Sweeney KJ and Kerin MJ. A Comparison of Surgical Impression, Histological Findings and Microbiological Results at Open Appendicectomy. I M J. 2007;100(7): 593-6.
16. Aydin OzgĂĽr. Incidental parasitic infestations in surgically removed appendices: a retrospective analysis. Diagnostic Pathology. 2007;2:6. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
17. Thanikachalam MP, Kasemsuk Y, Mak JW, Sharifah E, Sand Kandasamy P. A study of parasitic infections in the luminal contents and tissue sections of appendix specimens. Trop Biomed. 2008;25(2):166-172. [Pubmed]   
18. Jones RN, Romberg PR, Varnam DJ, Mafhi D. A comparison of the antimicrobial activity of selected broad spectrum antimicrobial tested against multi-drug resistant Gram-negative studies for the MYSTIC programmer in India. Int J Anti-Microbs Agent. 2002;20(6):426-31. [DOI via Crossref]   
19. Jones PF. Suspected acute appendicitis trends in management over the past 30 years. Br J Surg. 2001;88:1570-7. [DOI via Crossref]    [Pubmed]   
20. Gladman MA, Knowles CH, Gladman LJ, Payne JG. Intra-operative culture in appendicitis: traditional practice challenged. Ann R Coll Surg Engl. 2004;86(3):196-201. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
21. Fatemeh N. Histopathological Feature of Acute Appendicitis in Kerman-Iran from 1997 to 2003 American J. Environmental Sci .2005;1(2):130-2. [DOI via Crossref]   
22. Tariq W Khanzada, Abdul Samad and Champa Sushel. Negative Appendectomy Rate: Can It Be Reduced? JLUMH. 2009;8(1):19-22.
23. Herd ME, Cross PA, Dutt S. Histological audit of acute appendicitis. J Clin Path. 1992;45:456-8. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
24. Ozel SK, Ilhan N, Kazez A, Apak S, Ilhan N. Is urinary 5-HIAA determination a valuable method in diagnosis of acute appendicitis in children? J Indian Assoc Pediatr Surg. 2006;11:35-8. [DOI via Crossref]   
25. Rordam P, Mortensen P, Hindberg I, Christiansen J. Acute appendicitis and plasma concentration of serotonin. Acta Chir Scand. 1987;153:437-9. [Pubmed]   
26. Kalra U, Chitkara N, Dadoo RC, Singh GP, Gulati P, Narula S. Evaluation of plasma serotonin concentration in acute appendicitis. Indian J Gastroenterol. 1997;16:18-9. [Pubmed]   
27. Ilkhanizadeh B, Owji AA, Tavangar SM, Vasei M, Tabei SM. Spot urine 5-hydroxy indole acetic acid and acute appendicitis. Hepatogastroenterology. 2001;48(39):609-13. [Pubmed]   
28. Oruc MT, Kulah B, Ozozan O, Ozer V, Kulacoglu H, Turhan T. The value of 5-hydroxy indole acetic acid measurement in spot urine in diagnosis of acute appendicitis. East Afr Med J. 2004;81:40-1. [DOI via Crossref]    [Pubmed]   
29. Hernandez R, J Ashok, Rosiere L, Henderson S O. A prospective clinical trial evaluating urinary 5-hydroxyindoleacetic acid levels in the diagnosis of acute appendicitis. AmJ Emerg Med. 2008;26(3):282-6. [DOI via Crossref]    [Pubmed]   

This Article Cited By the following articles

Biomarkers of acute appendicitis: systematic review and cost–benefit trade-off analysis
Surg Endosc 2017; 31(3): 1022.

1
 
Spot urinary 5-hydroxyindoleacetic acid is not an ideal diagnostic test for acute appendicitis
The American Journal of Emergency Medicine 2016; 34(9): 1750.

2
 
Does elevated urinary 5-hydroxyindole acetic acid level predict acute appendicitis in children?
Emerg Med J 2016; 33(12): 848.

3
 
How to Cite this Article
Pubmed Style

Hamid Hindi Sarhan, Aseel Fawzi Hatroosh, Amina Hamed Alobaidi. The role of urinary 5- hydroxyindoleacetic acid determination in diagnosis of acute appendicitis. A J Physiol Biochem Pharmacol. 2013; 2(1): 1-5. doi:10.5455/jib.20121026084836


Web Style

Hamid Hindi Sarhan, Aseel Fawzi Hatroosh, Amina Hamed Alobaidi. The role of urinary 5- hydroxyindoleacetic acid determination in diagnosis of acute appendicitis. http://www.ajpbp.com/?mno=20622 [Access: November 18, 2018]. doi:10.5455/jib.20121026084836


AMA (American Medical Association) Style

Hamid Hindi Sarhan, Aseel Fawzi Hatroosh, Amina Hamed Alobaidi. The role of urinary 5- hydroxyindoleacetic acid determination in diagnosis of acute appendicitis. A J Physiol Biochem Pharmacol. 2013; 2(1): 1-5. doi:10.5455/jib.20121026084836



Vancouver/ICMJE Style

Hamid Hindi Sarhan, Aseel Fawzi Hatroosh, Amina Hamed Alobaidi. The role of urinary 5- hydroxyindoleacetic acid determination in diagnosis of acute appendicitis. A J Physiol Biochem Pharmacol. (2013), [cited November 18, 2018]; 2(1): 1-5. doi:10.5455/jib.20121026084836



Harvard Style

Hamid Hindi Sarhan, Aseel Fawzi Hatroosh, Amina Hamed Alobaidi (2013) The role of urinary 5- hydroxyindoleacetic acid determination in diagnosis of acute appendicitis. A J Physiol Biochem Pharmacol, 2 (1), 1-5. doi:10.5455/jib.20121026084836



Turabian Style

Hamid Hindi Sarhan, Aseel Fawzi Hatroosh, Amina Hamed Alobaidi. 2013. The role of urinary 5- hydroxyindoleacetic acid determination in diagnosis of acute appendicitis. American Journal of Physiology, Biochemistry and Pharmacology, 2 (1), 1-5. doi:10.5455/jib.20121026084836



Chicago Style

Hamid Hindi Sarhan, Aseel Fawzi Hatroosh, Amina Hamed Alobaidi. "The role of urinary 5- hydroxyindoleacetic acid determination in diagnosis of acute appendicitis." American Journal of Physiology, Biochemistry and Pharmacology 2 (2013), 1-5. doi:10.5455/jib.20121026084836



MLA (The Modern Language Association) Style

Hamid Hindi Sarhan, Aseel Fawzi Hatroosh, Amina Hamed Alobaidi. "The role of urinary 5- hydroxyindoleacetic acid determination in diagnosis of acute appendicitis." American Journal of Physiology, Biochemistry and Pharmacology 2.1 (2013), 1-5. Print. doi:10.5455/jib.20121026084836



APA (American Psychological Association) Style

Hamid Hindi Sarhan, Aseel Fawzi Hatroosh, Amina Hamed Alobaidi (2013) The role of urinary 5- hydroxyindoleacetic acid determination in diagnosis of acute appendicitis. American Journal of Physiology, Biochemistry and Pharmacology, 2 (1), 1-5. doi:10.5455/jib.20121026084836





Most Viewed Articles
  • Slippery Elm, its Biochemistry, and use as a Complementary and Alternative Treatment for Laryngeal Irritation
    Christopher R Watts, Bernard rousseau
    J Investig Biochem. 2012; 1(1): 17-23
    » Abstract & References » doi: 10.5455/jib.20120417052415

  • Selected disease fundamentals based on the unified cell bioenergetics
    Andrzej Kasperski, Renata Kasperska
    J Investig Biochem. 2013; 2(2): 93-100
    » Abstract & References » doi: 10.5455/jib.20130227041230

  • Prevalence of vitamin B12 deficiency in Helicobacter pylori infected patients in Jordan.
    Saad Abed Aljalil Alfawaeir, Mohammad Basim Abuzaid
    J Investig Biochem. 2013; 2(1): 21-25
    » Abstract & References » doi: 10.5455/jib.20121215061954

  • Serum levels of high-sensitivity C-reactive protein (hs-CRP) in Helicobacter pylori infected patients
    Saad Al-Fawaeir, Mohammad Basim Abu Zaid
    J Investig Biochem. 2013; 2(1): 32-36
    » Abstract & References » doi: 10.5455/jib.20121225015606

  • Molecular Characterization of Alpha and Beta Thalassemia in Yemeni Sickle Cell Disease Children
    Saeed Thabet Nasher, Mohammed Wael Abu Ghoush
    J Investig Biochem. 2013; 2(1): 44-52
    » Abstract & References » doi: 10.5455/jib.20130105022014

  • Hepatoprotective and In vivo Anti-oxidant Activity of Costus afer Leaf Extracts against Acetaminophen-Induced Hepatotoxicity in Rats
    God'swill Nduka Anyasor, Kofoworola O. Odunsanya, Audrey C. Ibeneme
    J Investig Biochem. 2013; 2(1): 53-61
    » Abstract & References » doi: 10.5455/jib.20130301030851

  • Correlation Between Elevated Hepatic Enzymes & High-Sensitivity Crp in Metabolic Syndrome.
    Al-Rubaye Faisal, Shakir Mahmood, Jumah Kadhum
    J Investig Biochem. 2012; 1(1): 6-11
    » Abstract & References » doi: 10.5455/jib.20120528094732

  • Serum Lipid Profile of Breast Cancer Patients in Kashmir
    Showkat Ahmad Bhat, Manzoor R Mir, Sabhiya Majid, Ahmad Arif Reshi, Ishraq Husain, Tehseen Hassan, Hilal Ahmad
    J Investig Biochem. 2013; 2(1): 26-31
    » Abstract & References » doi: 10.5455/jib.20121125075314

  • Alteration of antioxidant enzymes and oxidative stress in elderly patient.
    Seema L. Jawalekar1, Anil K. Bhutey2, Aarti Karnik3, Vasant T. Surve4
    J Investig Biochem. 2013; 2(1): 37-43
    » Abstract & References » doi: 10.5455/jib.20130129093838

  • Potential Role of Oxidized LDL (ox-LDL) and Adhesion Molecules (VCAM-1, ICAM-1) in Type 2 Diabetes Mellitus Patients in Qassim region, KSA
    Manal Basyouni, Maha Ahmed, Hisham Ismail , Iman Esmat
    J Investig Biochem. 2012; 1(1): 48-54
    » Abstract & References » doi: 10.5455/jib.20120417052458

  • Most Downloaded
  • Slippery Elm, its Biochemistry, and use as a Complementary and Alternative Treatment for Laryngeal Irritation
    Christopher R Watts, Bernard rousseau
    J Investig Biochem. 2012; 1(1): 17-23
    » Abstract & References » doi: 10.5455/jib.20120417052415

  • Selected disease fundamentals based on the unified cell bioenergetics
    Andrzej Kasperski, Renata Kasperska
    J Investig Biochem. 2013; 2(2): 93-100
    » Abstract & References » doi: 10.5455/jib.20130227041230

  • Serum levels of high-sensitivity C-reactive protein (hs-CRP) in Helicobacter pylori infected patients
    Saad Al-Fawaeir, Mohammad Basim Abu Zaid
    J Investig Biochem. 2013; 2(1): 32-36
    » Abstract & References » doi: 10.5455/jib.20121225015606

  • Alteration of antioxidant enzymes and oxidative stress in elderly patient.
    Seema L. Jawalekar1, Anil K. Bhutey2, Aarti Karnik3, Vasant T. Surve4
    J Investig Biochem. 2013; 2(1): 37-43
    » Abstract & References » doi: 10.5455/jib.20130129093838

  • Serum Lipid Profile of Breast Cancer Patients in Kashmir
    Showkat Ahmad Bhat, Manzoor R Mir, Sabhiya Majid, Ahmad Arif Reshi, Ishraq Husain, Tehseen Hassan, Hilal Ahmad
    J Investig Biochem. 2013; 2(1): 26-31
    » Abstract & References » doi: 10.5455/jib.20121125075314

  • Hepatoprotective and In vivo Anti-oxidant Activity of Costus afer Leaf Extracts against Acetaminophen-Induced Hepatotoxicity in Rats
    God'swill Nduka Anyasor, Kofoworola O. Odunsanya, Audrey C. Ibeneme
    J Investig Biochem. 2013; 2(1): 53-61
    » Abstract & References » doi: 10.5455/jib.20130301030851

  • Evaluation of glycated albumin and microalbuminuria as early risk markers of nephropathy in type 2 diabetes mellitus
    Suresh Babu Kondaveeti, Ivvala Anand Shaker
    J Investig Biochem. 2013; 2(2): 127-131
    » Abstract & References » doi: 10.5455/jib.20130507061739

  • Comparative study of ischemia modified albumin and nitric oxide in hyperthyroidism
    Monica Verma1, Kiran Dahiya1, Veena Singh Ghalaut1, Abhishek Soni2, Jagjeet Singh3, Vasudha Dhupper1
    J Investig Biochem. 2014; 3(1): 17-20
    » Abstract & References » doi: 10.5455/jib.20130707090209

  • Prevalence of vitamin B12 deficiency in Helicobacter pylori infected patients in Jordan.
    Saad Abed Aljalil Alfawaeir, Mohammad Basim Abuzaid
    J Investig Biochem. 2013; 2(1): 21-25
    » Abstract & References » doi: 10.5455/jib.20121215061954

  • Serum lipid profile in Helicobacter pylori infected patients
    Saad Al-Fawaeir, Mohammad Abu Zaid, Ayman Abu Awad, Baker Alabedallat
    J Investig Biochem. 2013; 2(2): 132-135
    » Abstract & References » doi: 10.5455/jib.20130603084538

  • Most Cited Articles
  • Biochemical study on the impact of Nigella sativa and virgin olive oils on cadmium-induced nephrotoxicity and neurotoxicity in rats
    Eman Taha Mohammed, Khalid Shaban Hashem, Mahmoud Ramadan Abdel Rheim
    J Investig Biochem. 2014; 3(2): 71-78
    » Abstract & References » doi: 10.5455/jib.20140716041908
    Cited : 6 times [Click to see citing articles]

  • Study and Characterization of the Antioxidant Activity of the Inclusion Complex of Apigenin with β-Cyclodextrin and HP-β-Cyclodextrin in solution
    Jinxia Li
    J Investig Biochem. 2014; 3(3): 107-111
    » Abstract & References » doi: 10.5455/jib.20140905032504
    Cited : 5 times [Click to see citing articles]

  • Slippery Elm, its Biochemistry, and use as a Complementary and Alternative Treatment for Laryngeal Irritation
    Christopher R Watts, Bernard rousseau
    J Investig Biochem. 2012; 1(1): 17-23
    » Abstract & References » doi: 10.5455/jib.20120417052415
    Cited : 4 times [Click to see citing articles]

  • Alterations in plasma lipids, glutathione and homocysteine in relation to dietary copper in rats
    Magda A. Megahed1, Kamel M. A. Hassanin2, Ibrahim M. I. Youssef3, Abdul Baset A. Elfghi4, Kamal A. Amin2
    J Investig Biochem. 2014; 3(1): 21-25
    » Abstract & References » doi: 10.5455/jib.20130716075753
    Cited : 4 times [Click to see citing articles]

  • Quercetin, a natural phytochemical and antioxidant protects against sodium azide-induced hepatic and splenic oxidative stress in rats
    Oluwatobi Temitope Somade, Oluseyi Adeboye Akinloye, Michael Oluwayemi Adeyeye, Gbenga Daniel Fabunmi, Olakunle Opeoluwa Idowu, Funmilayo Oluwaseun Badmus, Barakat Oluwatoyin Salaudeen
    J Investig Biochem. 2015; 4(3): 69-74
    » Abstract & References » doi: 10.5455/jib.20151220014400
    Cited : 3 times [Click to see citing articles]

  • A New Marker For The Diagnosýs Of Sepsis: Presepsin
    Mehmet Agilli, Irfan Sener, Fatih Yesildal, Tevfik Honca, Ibrahim Aydin, Emin Ozgur Akgul, Halil Yaman
    J Investig Biochem. 2012; 1(1): 55-57
    » Abstract & References » doi: 10.5455/jib.20120521073837
    Cited : 3 times [Click to see citing articles]

  • The role of urinary 5- hydroxyindoleacetic acid determination in diagnosis of acute appendicitis
    Hamid Hindi Sarhan, Aseel Fawzi Hatroosh, Amina Hamed Alobaidi
    J Investig Biochem. 2013; 2(1): 1-5
    » Abstract & References » doi: 10.5455/jib.20121026084836
    Cited : 3 times [Click to see citing articles]

  • Serum Lipid Profile of Breast Cancer Patients in Kashmir
    Showkat Ahmad Bhat, Manzoor R Mir, Sabhiya Majid, Ahmad Arif Reshi, Ishraq Husain, Tehseen Hassan, Hilal Ahmad
    J Investig Biochem. 2013; 2(1): 26-31
    » Abstract & References » doi: 10.5455/jib.20121125075314
    Cited : 3 times [Click to see citing articles]

  • Phytochemical content of Cnidoscolus aconitifolius leaves and toxicological effect of its aqueous leaf extract in Wistar rats
    Doris Akachukwu, Polycarp Nnacheta Okafor, Chiedozie O Ibegbulem
    J Investig Biochem. 2014; 3(1): 26-31
    » Abstract & References » doi: 10.5455/jib.20140504023102
    Cited : 3 times [Click to see citing articles]

  • Myeloperoxidase, Malondialdehyde and serum Lipids in type 2 diabetes mellitus
    Mohamedi Begum, Ashok Kumar J., Hilda Priya D'Souza, Sushith Sushith, Prathima M.B, Reshma S., Shashikala M.D, Manjula Anil, Suriyan S Nair, Kavitha Ashok Kumar
    J Investig Biochem. 2015; 4(1): 13-17
    » Abstract & References » doi: 10.5455/jib.20150331115356
    Cited : 3 times [Click to see citing articles]