E-ISSN 2578-7322
 

Original Research 


Anti-inflammatory property and gas chromatography-mass spectrometry analysis of ethyl acetate fraction of Crateva adansonii DC leaves

Chineye C. Umeti, Funmilayo Onajobi, Efere M. Oboutor, Godswill N. Anyasor, Edward B. Esan.

Abstract
Aims: This study was designed to determine the most active anti-inflammatory fraction of C. adansonii leaves and characterize the bioactive compounds.
Methods: Dried and pulverized C. adansonii leaves (CAL) were extracted with 70% methanol, followed by successive solvent partitioning into hexane (CALH), ethyl acetate (CALE), butanol (CALB) and aqueous (CALA) leaf fractions. In vitro antioxidant assays carried out on the extract/fractions were DPPH, hydrogen peroxide, ferric reducing antioxidant power (FRAP), nitric oxide scavenging, total antioxidant capacity (TAC), total phenol (TP) and flavonoid (TF) assays. In vitro anti-inflammatory assays investigated were heat-induced bovine serum albumin (BSA) denaturation and human red blood cell (HRBC) membrane stabilization against hypotonicity- induced hemolysis. Formaldehyde-induced inflammation model in rats was carried out, followed by paw edema measurement as well as serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities assays. The ethyl acetate (CALE) fraction was subjected to GC-MS analytical method.
Results: CAL extract and fractions substantially inhibited BSA denaturation and stabilized HRBC membrane against hypotonicity-induced hemolysis, however CALE exhibited the highest activity. CALE also suppressed formaldehyde-induced rat paw edema and significantly (p<0.001) reduced serum ALT and AST activities. In addition, CALE demonstrated significantly high (P<0.05) antioxidant activities in DPPH and nitric oxide scavenging activities when compared with the other test samples. CALE also exhibited an appreciable amount in FRAP, TAC, and TF. GC-MS analysis of CALE indicated the presence of 19 tentatively identified compounds with anti-inflammatory and/or antioxidant properties.
Conclusion: Ethyl acetate fraction of C. adansonii leaves exhibited anti-inflammatory activity which could be through antioxidant effect and perhaps suppression of pro-inflammatory mediators. Hence, ethyl acetate fraction of C. adansonii leaf could serve as an important source of lead drug candidate for pharmaceutical drug development in the management of inflammation.

Key words: Anti-inflammatory; Antioxidant; Arthritis; Bioactive compounds; Crateva adansonii


 
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Pubmed Style

Umeti CC, Onajobi F, Oboutor EM, Anyasor GN, Esan EB, . Anti-inflammatory property and gas chromatography-mass spectrometry analysis of ethyl acetate fraction of Crateva adansonii DC leaves. A J Physiol Biochem Pharmacol. 2019; 9(1): 9-20. doi:10.5455/ajpbp.20181226090820


Web Style

Umeti CC, Onajobi F, Oboutor EM, Anyasor GN, Esan EB, . Anti-inflammatory property and gas chromatography-mass spectrometry analysis of ethyl acetate fraction of Crateva adansonii DC leaves. http://www.ajpbp.com/?mno=23270 [Access: March 22, 2019]. doi:10.5455/ajpbp.20181226090820


AMA (American Medical Association) Style

Umeti CC, Onajobi F, Oboutor EM, Anyasor GN, Esan EB, . Anti-inflammatory property and gas chromatography-mass spectrometry analysis of ethyl acetate fraction of Crateva adansonii DC leaves. A J Physiol Biochem Pharmacol. 2019; 9(1): 9-20. doi:10.5455/ajpbp.20181226090820



Vancouver/ICMJE Style

Umeti CC, Onajobi F, Oboutor EM, Anyasor GN, Esan EB, . Anti-inflammatory property and gas chromatography-mass spectrometry analysis of ethyl acetate fraction of Crateva adansonii DC leaves. A J Physiol Biochem Pharmacol. (2019), [cited March 22, 2019]; 9(1): 9-20. doi:10.5455/ajpbp.20181226090820



Harvard Style

Umeti, C. C., Onajobi, . F., Oboutor, . E. M., Anyasor, . G. N., Esan, . E. B. & (2019) Anti-inflammatory property and gas chromatography-mass spectrometry analysis of ethyl acetate fraction of Crateva adansonii DC leaves. A J Physiol Biochem Pharmacol, 9 (1), 9-20. doi:10.5455/ajpbp.20181226090820



Turabian Style

Umeti, Chineye C., Funmilayo Onajobi, Efere M. Oboutor, Godswill N. Anyasor, Edward B. Esan, and . 2019. Anti-inflammatory property and gas chromatography-mass spectrometry analysis of ethyl acetate fraction of Crateva adansonii DC leaves. American Journal of Physiology, Biochemistry and Pharmacology, 9 (1), 9-20. doi:10.5455/ajpbp.20181226090820



Chicago Style

Umeti, Chineye C., Funmilayo Onajobi, Efere M. Oboutor, Godswill N. Anyasor, Edward B. Esan, and . "Anti-inflammatory property and gas chromatography-mass spectrometry analysis of ethyl acetate fraction of Crateva adansonii DC leaves." American Journal of Physiology, Biochemistry and Pharmacology 9 (2019), 9-20. doi:10.5455/ajpbp.20181226090820



MLA (The Modern Language Association) Style

Umeti, Chineye C., Funmilayo Onajobi, Efere M. Oboutor, Godswill N. Anyasor, Edward B. Esan, and . "Anti-inflammatory property and gas chromatography-mass spectrometry analysis of ethyl acetate fraction of Crateva adansonii DC leaves." American Journal of Physiology, Biochemistry and Pharmacology 9.1 (2019), 9-20. Print. doi:10.5455/ajpbp.20181226090820



APA (American Psychological Association) Style

Umeti, C. C., Onajobi, . F., Oboutor, . E. M., Anyasor, . G. N., Esan, . E. B. & (2019) Anti-inflammatory property and gas chromatography-mass spectrometry analysis of ethyl acetate fraction of Crateva adansonii DC leaves. American Journal of Physiology, Biochemistry and Pharmacology, 9 (1), 9-20. doi:10.5455/ajpbp.20181226090820





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