E-ISSN 2578-7322
 

Original Research 


Some conventional and non-conventional hematological and biochemical markers for characterizing some acute and chronic presentations of sickle cell patients.

Kwabena Nsiah, Victoria Pearl Arthur, Daniel Ansong, Alex Osei-Akoto, Dela Ocloo.

Abstract
Background
Some acute and chronic presentations of sickle cell disease (SCD) are splenomegaly, acute chest syndrome, priapism and leg ulceration. Hematological and biochemical parameters are used for diagnosis and prognosis.

Method
In this cross-sectional study, carried out over a 12-month period, involving 291 patients, some conventional hematological and biochemical parameters, and two non-conventional determinations, AST:ALT ratio and urine color, were used to characterize the patients with some selected clinical presentations.

Results
For these subjects studied (n = 21), the mean values of hemoglobin (Hb), red blood cells (RBC), white blood cells, (WBC), reticulocytes, platelets and fetal hemoglobin (HbF) were 6.6 g/dl, 2.28×1012/L; 16.3 ×109/L, 27.1%, 310.1×109/L and 1.84%, respectively. The mean AST:ALT ratio was 2.058 and the urine color, 2.381. These values were compared to 5 non-anemic counterparts, who had the following mean values of the parameters; Hb = 11.9 g/dl; RBC = 4.13×1012/L WBC = 7.2×109/L; reticulocytess = 5.2%; platelets = 282.4×109/L; HbF = 1.48%. A Mann-Whitney non parametric two-tailed test showed several of the differences between the patients with complications and the non-anemic, were statistically significant (p< 0.05). The subjects with the complications were moderately anemic, having elevated levels of lactate dehydrogenase, total bilirubin, indirect bilirubin and AST:ALT ratio.

Conclusion
The four clinical presentations were characterized by anemia, accompanied by high levels of conventional markers, like reticulocytes, LDH and bilirubin, together with an elevated AST:ALT ratio, a non-conventional marker.

Key words: Sickle cell, hemoglobinopathy, hemolytic, markers, conventional, non-conventional


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Kwabena Nsiah
Articles by Victoria Pearl Arthur
Articles by Daniel Ansong
Articles by Alex Osei-Akoto
Articles by Dela Ocloo
on Google
on Google Scholar
Article Statistics
 Viewed: 2424
Downloaded: 590
Cited: 0

REFERENCES
1. World Health Organisation. (1994) Guidelines for the Control of Haemoglobin Disorders. Geneva, Switzerland: WHO.
2. Steinberg MH, Brugnara C. Pathophysiologically-based approaches to treatment of sickle cell disease. Annu Rev Med. 2003;54:89-112. [DOI via Crossref]    [Pubmed]   
3. Christoph GW, Hofrichter J, Eaton WA. Understanding the shape of sickled red cells. Biophys J. 2005;88:1371-6. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
4. Kam, Z, Hofrichter, J. Quasi-elastic laser light scattering from solutions and gels of HbS. Biophys J. 1986;50:1015-20. [DOI via Crossref]   
5. Bunn HF. Human hemoglobins: normal and abnormal: methemoglobinemia. In: Hematology of Infancy and Childhood. Nathan, D.G. and Oski, F.A. (eds.) 4th edition, WB Saunders, Philadelphia, 1993; pp 698-731.
6. Noguchi CT, Holey JD, Abraham DJ, Schechter, AN. Inhibition of sickle hemoglobin polymerization as a basis for the therapeutic approaches to sickle cell anemia. In: Burger's Medicinal Chemistry volume III, Abraham, D.J.(ed.) Wiley and Sons, New York 2003; pp 443-479. [DOI via Crossref]   
7. Levasseur DN, Ryan TM, Pawlik KM, Townes TM. Correction of a mouse model of sickle cell disease: lentivirus/antisickling &#946;-globin gene transduction of unmobilized, purified hematopoietic cells. Blood. 2003;102:4312-9. [DOI via Crossref]    [Pubmed]   
8. Kato GJ, Martyr S, Blackwelder WC, Nichols JS, Coles WA, Hunter LA. et al. Level of soluble endothelium-derived adhesive molecules in patients with sickle cell disease are associated with pulmonary hypertension, organ dysfunction and mortality. Br J Haematol. 2005;130:943-53. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
9. Kato GJ, Gladwin MT, Steinberg MH. Deconstructing sickle cell disease: reappraisal of the role of hemolysis in the development of clinical subphenotypes. Blood Rev. 2007;21:37-47. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
10. Morris CR. Vascular risk assessment in patients with sickle cell disease. Haematologica. 2011;96:1-5. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
11. Nsiah K, Dzogbefia VP, Ansong D, Boateng H, Ocloo D, Osei Akoto A. The pattern of AST and ALT changes in relation to haemolysis in sickle cell disease Clinical Medicine Insights; Blood Disorders. 2011;4:1-9.
12. Singer K, Chernoff AI, Singer L. Studies on abnormal hemoglobins: Their denaturation in sickle cell anemia and other hematologic disorders by means of alkali denaturation. Blood. 1951;6:413-28. [Pubmed]   
13. Konotey-Ahulu FID. The Sickle Cell Disease Patient: 1st ed. Macmillan, 1991; pp:341-8.
14. Edington GM. Pathology of malaria in west Africa. Br J Med. 1967;1:715-21. [DOI via Crossref]   
15. Koshy M, Entsuah R, Koranda A, Kraus AP, Johnson R, Bellvue R. et al. Leg ulcers in patients with sickle cell disease. Blood 1989;74:1403-8. [Pubmed]   
16. Akinyanju O, Akinsete I. Leg ulceration in sickle cell disease in Nigeria. Trop Geogr Med. 1979;31:87-91. [Pubmed]   
17. Ahmed SG, Ibrahim UA, Hassan AW. Haematological parameters in sickle cell anemia patients with and without priapism. Ann Saudi Med. 2006;26:439-43. [Pubmed]   
18. Adedeji MO, Onuora VC, Ukoli FAM. Haematological parameters associated with priapism in Nigerian patients with homozygous sickle cell disease. J Trop Med Hyg. 1988;96:157-9.
19. Bunn HF, Nathan DG, Dover GJ, Hebbel RP, Platt OS, Rosse WF, Ware RE. Pulmonary hypertension and nitric oxide depletion in sickle cell disease. Blood. 2010;116:687-92. [DOI via Crossref]    [Pubmed]   
20. Krajewski ML, Hsu LL, Gladwin MT. The proverbial chicken or egg? Dissection of the role of cell-free hemoglobin versus reactive oxygen species in sickle cell pathophysiology. Am J Physiol Heart Circ Physiol. 2008;295:H4-H7. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
21. Hsu LL, Champion HC, Campbell-Lee SA, Biralacqua TJ, Manci EA, Diuran BA et al. Hemolysis in sickle cell mice causes pulmonary hypertension due to global impairment in nitric oxide bioavailability. Blood 2007;109:3088-98. [Pubmed]    [PMC Free Fulltext]   
22. Nolan VG, Wyszynski DF, Farrer LA, Steinberg MH. Hemolysis associated priapism in sickle cell disease. Blood. 2005;106:3264-7. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
23. Nolan VG, Adewoye A, Baldwin C, Wang L, Ma Q, Wyszynski DF et al. Sickle cell leg ulcers: associations with haemolysis and SNPs in Klotho, TEK and genes of the TGF-beta/BMP pathway. Br J Haematol. 2006;133:570-8. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
24. Fevery J, Verwilghen R, Tan TG, De Groote J. Glucuronidation of bilirubin and occurrence of pigment gallstones in patients with chronic haemolytic disease. Eur J Clin Invest. 1980;10:219-26. [DOI via Crossref]    [Pubmed]   
25. Brown AK, Sleeper LA, Scott T, Miller CH, Pegelow Gill FM. Waclawiw MA. Reference values and hematologic changes from birth to 5 years in patients with sickle cell disease. Arch Pediatr Adolesc Med. 1994;148:796-804. [DOI via Crossref]    [Pubmed]   
26. Johnson CS, Omata M, Tong MJ, Simmons JF Jr., Weiner J, Tatter D. Liver involvement in sickle cell disease. Medicine. 1985;64:349-56. [DOI via Crossref]    [Pubmed]   
27. Solanki DL, Kletter GG, Castro O. Acute splenic sequestration crises in adults with sickle cell disease. Am J Med. 1986;80:985-90. [DOI via Crossref]   
28. Aquino VM, Norvell JM, Buchanan GR. Acute splenic complications in children with sickle cell-hemoglobin C disease. The Journal of Pediatrics. 1997;130:961-5. [DOI via Crossref]   
29. Okuonghae HO, Nwankwo MU, Offor E. 166 episodes of fever in 162 children with sickle cell disease aged six months to 16 years. J Trop Pediatr. 1992;38:83-5. [DOI via Crossref]    [Pubmed]   

How to Cite this Article
Pubmed Style

Kwabena Nsiah, Victoria Pearl Arthur, Daniel Ansong, Alex Osei-Akoto, Dela Ocloo. Some conventional and non-conventional hematological and biochemical markers for characterizing some acute and chronic presentations of sickle cell patients.. A J Physiol Biochem Pharmacol. 2013; 2(1): 14-20. doi:10.5455/jib.20121217043354


Web Style

Kwabena Nsiah, Victoria Pearl Arthur, Daniel Ansong, Alex Osei-Akoto, Dela Ocloo. Some conventional and non-conventional hematological and biochemical markers for characterizing some acute and chronic presentations of sickle cell patients.. http://www.ajpbp.com/?mno=25695 [Access: November 18, 2018]. doi:10.5455/jib.20121217043354


AMA (American Medical Association) Style

Kwabena Nsiah, Victoria Pearl Arthur, Daniel Ansong, Alex Osei-Akoto, Dela Ocloo. Some conventional and non-conventional hematological and biochemical markers for characterizing some acute and chronic presentations of sickle cell patients.. A J Physiol Biochem Pharmacol. 2013; 2(1): 14-20. doi:10.5455/jib.20121217043354



Vancouver/ICMJE Style

Kwabena Nsiah, Victoria Pearl Arthur, Daniel Ansong, Alex Osei-Akoto, Dela Ocloo. Some conventional and non-conventional hematological and biochemical markers for characterizing some acute and chronic presentations of sickle cell patients.. A J Physiol Biochem Pharmacol. (2013), [cited November 18, 2018]; 2(1): 14-20. doi:10.5455/jib.20121217043354



Harvard Style

Kwabena Nsiah, Victoria Pearl Arthur, Daniel Ansong, Alex Osei-Akoto, Dela Ocloo (2013) Some conventional and non-conventional hematological and biochemical markers for characterizing some acute and chronic presentations of sickle cell patients.. A J Physiol Biochem Pharmacol, 2 (1), 14-20. doi:10.5455/jib.20121217043354



Turabian Style

Kwabena Nsiah, Victoria Pearl Arthur, Daniel Ansong, Alex Osei-Akoto, Dela Ocloo. 2013. Some conventional and non-conventional hematological and biochemical markers for characterizing some acute and chronic presentations of sickle cell patients.. American Journal of Physiology, Biochemistry and Pharmacology, 2 (1), 14-20. doi:10.5455/jib.20121217043354



Chicago Style

Kwabena Nsiah, Victoria Pearl Arthur, Daniel Ansong, Alex Osei-Akoto, Dela Ocloo. "Some conventional and non-conventional hematological and biochemical markers for characterizing some acute and chronic presentations of sickle cell patients.." American Journal of Physiology, Biochemistry and Pharmacology 2 (2013), 14-20. doi:10.5455/jib.20121217043354



MLA (The Modern Language Association) Style

Kwabena Nsiah, Victoria Pearl Arthur, Daniel Ansong, Alex Osei-Akoto, Dela Ocloo. "Some conventional and non-conventional hematological and biochemical markers for characterizing some acute and chronic presentations of sickle cell patients.." American Journal of Physiology, Biochemistry and Pharmacology 2.1 (2013), 14-20. Print. doi:10.5455/jib.20121217043354



APA (American Psychological Association) Style

Kwabena Nsiah, Victoria Pearl Arthur, Daniel Ansong, Alex Osei-Akoto, Dela Ocloo (2013) Some conventional and non-conventional hematological and biochemical markers for characterizing some acute and chronic presentations of sickle cell patients.. American Journal of Physiology, Biochemistry and Pharmacology, 2 (1), 14-20. doi:10.5455/jib.20121217043354





Most Viewed Articles
  • Slippery Elm, its Biochemistry, and use as a Complementary and Alternative Treatment for Laryngeal Irritation
    Christopher R Watts, Bernard rousseau
    J Investig Biochem. 2012; 1(1): 17-23
    » Abstract & References » doi: 10.5455/jib.20120417052415

  • Selected disease fundamentals based on the unified cell bioenergetics
    Andrzej Kasperski, Renata Kasperska
    J Investig Biochem. 2013; 2(2): 93-100
    » Abstract & References » doi: 10.5455/jib.20130227041230

  • Prevalence of vitamin B12 deficiency in Helicobacter pylori infected patients in Jordan.
    Saad Abed Aljalil Alfawaeir, Mohammad Basim Abuzaid
    J Investig Biochem. 2013; 2(1): 21-25
    » Abstract & References » doi: 10.5455/jib.20121215061954

  • Serum levels of high-sensitivity C-reactive protein (hs-CRP) in Helicobacter pylori infected patients
    Saad Al-Fawaeir, Mohammad Basim Abu Zaid
    J Investig Biochem. 2013; 2(1): 32-36
    » Abstract & References » doi: 10.5455/jib.20121225015606

  • Molecular Characterization of Alpha and Beta Thalassemia in Yemeni Sickle Cell Disease Children
    Saeed Thabet Nasher, Mohammed Wael Abu Ghoush
    J Investig Biochem. 2013; 2(1): 44-52
    » Abstract & References » doi: 10.5455/jib.20130105022014

  • Hepatoprotective and In vivo Anti-oxidant Activity of Costus afer Leaf Extracts against Acetaminophen-Induced Hepatotoxicity in Rats
    God'swill Nduka Anyasor, Kofoworola O. Odunsanya, Audrey C. Ibeneme
    J Investig Biochem. 2013; 2(1): 53-61
    » Abstract & References » doi: 10.5455/jib.20130301030851

  • Correlation Between Elevated Hepatic Enzymes & High-Sensitivity Crp in Metabolic Syndrome.
    Al-Rubaye Faisal, Shakir Mahmood, Jumah Kadhum
    J Investig Biochem. 2012; 1(1): 6-11
    » Abstract & References » doi: 10.5455/jib.20120528094732

  • Serum Lipid Profile of Breast Cancer Patients in Kashmir
    Showkat Ahmad Bhat, Manzoor R Mir, Sabhiya Majid, Ahmad Arif Reshi, Ishraq Husain, Tehseen Hassan, Hilal Ahmad
    J Investig Biochem. 2013; 2(1): 26-31
    » Abstract & References » doi: 10.5455/jib.20121125075314

  • Alteration of antioxidant enzymes and oxidative stress in elderly patient.
    Seema L. Jawalekar1, Anil K. Bhutey2, Aarti Karnik3, Vasant T. Surve4
    J Investig Biochem. 2013; 2(1): 37-43
    » Abstract & References » doi: 10.5455/jib.20130129093838

  • Potential Role of Oxidized LDL (ox-LDL) and Adhesion Molecules (VCAM-1, ICAM-1) in Type 2 Diabetes Mellitus Patients in Qassim region, KSA
    Manal Basyouni, Maha Ahmed, Hisham Ismail , Iman Esmat
    J Investig Biochem. 2012; 1(1): 48-54
    » Abstract & References » doi: 10.5455/jib.20120417052458

  • Most Downloaded
  • Slippery Elm, its Biochemistry, and use as a Complementary and Alternative Treatment for Laryngeal Irritation
    Christopher R Watts, Bernard rousseau
    J Investig Biochem. 2012; 1(1): 17-23
    » Abstract & References » doi: 10.5455/jib.20120417052415

  • Selected disease fundamentals based on the unified cell bioenergetics
    Andrzej Kasperski, Renata Kasperska
    J Investig Biochem. 2013; 2(2): 93-100
    » Abstract & References » doi: 10.5455/jib.20130227041230

  • Serum levels of high-sensitivity C-reactive protein (hs-CRP) in Helicobacter pylori infected patients
    Saad Al-Fawaeir, Mohammad Basim Abu Zaid
    J Investig Biochem. 2013; 2(1): 32-36
    » Abstract & References » doi: 10.5455/jib.20121225015606

  • Alteration of antioxidant enzymes and oxidative stress in elderly patient.
    Seema L. Jawalekar1, Anil K. Bhutey2, Aarti Karnik3, Vasant T. Surve4
    J Investig Biochem. 2013; 2(1): 37-43
    » Abstract & References » doi: 10.5455/jib.20130129093838

  • Serum Lipid Profile of Breast Cancer Patients in Kashmir
    Showkat Ahmad Bhat, Manzoor R Mir, Sabhiya Majid, Ahmad Arif Reshi, Ishraq Husain, Tehseen Hassan, Hilal Ahmad
    J Investig Biochem. 2013; 2(1): 26-31
    » Abstract & References » doi: 10.5455/jib.20121125075314

  • Hepatoprotective and In vivo Anti-oxidant Activity of Costus afer Leaf Extracts against Acetaminophen-Induced Hepatotoxicity in Rats
    God'swill Nduka Anyasor, Kofoworola O. Odunsanya, Audrey C. Ibeneme
    J Investig Biochem. 2013; 2(1): 53-61
    » Abstract & References » doi: 10.5455/jib.20130301030851

  • Evaluation of glycated albumin and microalbuminuria as early risk markers of nephropathy in type 2 diabetes mellitus
    Suresh Babu Kondaveeti, Ivvala Anand Shaker
    J Investig Biochem. 2013; 2(2): 127-131
    » Abstract & References » doi: 10.5455/jib.20130507061739

  • Comparative study of ischemia modified albumin and nitric oxide in hyperthyroidism
    Monica Verma1, Kiran Dahiya1, Veena Singh Ghalaut1, Abhishek Soni2, Jagjeet Singh3, Vasudha Dhupper1
    J Investig Biochem. 2014; 3(1): 17-20
    » Abstract & References » doi: 10.5455/jib.20130707090209

  • Prevalence of vitamin B12 deficiency in Helicobacter pylori infected patients in Jordan.
    Saad Abed Aljalil Alfawaeir, Mohammad Basim Abuzaid
    J Investig Biochem. 2013; 2(1): 21-25
    » Abstract & References » doi: 10.5455/jib.20121215061954

  • Serum lipid profile in Helicobacter pylori infected patients
    Saad Al-Fawaeir, Mohammad Abu Zaid, Ayman Abu Awad, Baker Alabedallat
    J Investig Biochem. 2013; 2(2): 132-135
    » Abstract & References » doi: 10.5455/jib.20130603084538

  • Most Cited Articles
  • Biochemical study on the impact of Nigella sativa and virgin olive oils on cadmium-induced nephrotoxicity and neurotoxicity in rats
    Eman Taha Mohammed, Khalid Shaban Hashem, Mahmoud Ramadan Abdel Rheim
    J Investig Biochem. 2014; 3(2): 71-78
    » Abstract & References » doi: 10.5455/jib.20140716041908
    Cited : 6 times [Click to see citing articles]

  • Study and Characterization of the Antioxidant Activity of the Inclusion Complex of Apigenin with β-Cyclodextrin and HP-β-Cyclodextrin in solution
    Jinxia Li
    J Investig Biochem. 2014; 3(3): 107-111
    » Abstract & References » doi: 10.5455/jib.20140905032504
    Cited : 5 times [Click to see citing articles]

  • Slippery Elm, its Biochemistry, and use as a Complementary and Alternative Treatment for Laryngeal Irritation
    Christopher R Watts, Bernard rousseau
    J Investig Biochem. 2012; 1(1): 17-23
    » Abstract & References » doi: 10.5455/jib.20120417052415
    Cited : 4 times [Click to see citing articles]

  • Alterations in plasma lipids, glutathione and homocysteine in relation to dietary copper in rats
    Magda A. Megahed1, Kamel M. A. Hassanin2, Ibrahim M. I. Youssef3, Abdul Baset A. Elfghi4, Kamal A. Amin2
    J Investig Biochem. 2014; 3(1): 21-25
    » Abstract & References » doi: 10.5455/jib.20130716075753
    Cited : 4 times [Click to see citing articles]

  • Quercetin, a natural phytochemical and antioxidant protects against sodium azide-induced hepatic and splenic oxidative stress in rats
    Oluwatobi Temitope Somade, Oluseyi Adeboye Akinloye, Michael Oluwayemi Adeyeye, Gbenga Daniel Fabunmi, Olakunle Opeoluwa Idowu, Funmilayo Oluwaseun Badmus, Barakat Oluwatoyin Salaudeen
    J Investig Biochem. 2015; 4(3): 69-74
    » Abstract & References » doi: 10.5455/jib.20151220014400
    Cited : 3 times [Click to see citing articles]

  • A New Marker For The Diagnosýs Of Sepsis: Presepsin
    Mehmet Agilli, Irfan Sener, Fatih Yesildal, Tevfik Honca, Ibrahim Aydin, Emin Ozgur Akgul, Halil Yaman
    J Investig Biochem. 2012; 1(1): 55-57
    » Abstract & References » doi: 10.5455/jib.20120521073837
    Cited : 3 times [Click to see citing articles]

  • The role of urinary 5- hydroxyindoleacetic acid determination in diagnosis of acute appendicitis
    Hamid Hindi Sarhan, Aseel Fawzi Hatroosh, Amina Hamed Alobaidi
    J Investig Biochem. 2013; 2(1): 1-5
    » Abstract & References » doi: 10.5455/jib.20121026084836
    Cited : 3 times [Click to see citing articles]

  • Serum Lipid Profile of Breast Cancer Patients in Kashmir
    Showkat Ahmad Bhat, Manzoor R Mir, Sabhiya Majid, Ahmad Arif Reshi, Ishraq Husain, Tehseen Hassan, Hilal Ahmad
    J Investig Biochem. 2013; 2(1): 26-31
    » Abstract & References » doi: 10.5455/jib.20121125075314
    Cited : 3 times [Click to see citing articles]

  • Phytochemical content of Cnidoscolus aconitifolius leaves and toxicological effect of its aqueous leaf extract in Wistar rats
    Doris Akachukwu, Polycarp Nnacheta Okafor, Chiedozie O Ibegbulem
    J Investig Biochem. 2014; 3(1): 26-31
    » Abstract & References » doi: 10.5455/jib.20140504023102
    Cited : 3 times [Click to see citing articles]

  • Myeloperoxidase, Malondialdehyde and serum Lipids in type 2 diabetes mellitus
    Mohamedi Begum, Ashok Kumar J., Hilda Priya D'Souza, Sushith Sushith, Prathima M.B, Reshma S., Shashikala M.D, Manjula Anil, Suriyan S Nair, Kavitha Ashok Kumar
    J Investig Biochem. 2015; 4(1): 13-17
    » Abstract & References » doi: 10.5455/jib.20150331115356
    Cited : 3 times [Click to see citing articles]