E-ISSN 2578-7322
 

Original Research 


Formulated hexane fraction of Costus afer leaves balm suppressed xylene induced topical inflammation in rat model

Godswill Nduka Anyasor, Olamidotun Henry Ijituyi.

Abstract
Objective: This study was designed to evaluate the effects of topically applied hexane fraction and aqueous extract of C. afer leaves balm and cream formula on xylene-induced ear edema.
Methods: In vitro anti-inflammatory study was carried out using thermally-induced protein denaturation and human erythrocyte membrane stabilization assays while in vivo anti-inflammatory study was performed using xylene-induced ear edema in rat model.
Results: Hexane fraction of C. afer leaves significantly inhibited thermally-induced protein denaturation and stabilized human erythrocyte membrane against hypotonicity-induced hemolysis when compared with aqueous extract of C. afer leaves. Furthermore, in vivo study showed that 10% and 20% w/w hexane fraction of C. afer leaves balm formula at 360 min significantly suppressed xylene-induced ear edema by 82.02% (0.16 ± 0.01 mm) and 96.62% (0.03 ± 0.01 mm) respectively when compared with 10% and 20% w/w aqueous extract of C. afer leaves cream formula with 55.06% (0.40 ± 0.01 mm) and 67.42% (0.29 ± 0.02 mm) inhibitions respectively.
Conclusion: The findings from this study revealed that hexane fraction of C. afer leaves balm formula exhibited higher anti-inflammatory activity against xylene-induced topical inflammation.

Key words: Anti-inflammation; balm; cream; Costus afer; topical inflammation


 
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Pubmed Style

Godswill Nduka Anyasor, Olamidotun Henry Ijituyi. Formulated hexane fraction of Costus afer leaves balm suppressed xylene induced topical inflammation in rat model. A J Physiol Biochem Pharmacol. 2018; 7(2): 54-60. doi:10.5455/ajpbp.20180518112401


Web Style

Godswill Nduka Anyasor, Olamidotun Henry Ijituyi. Formulated hexane fraction of Costus afer leaves balm suppressed xylene induced topical inflammation in rat model. http://www.ajpbp.com/?mno=299550 [Access: May 26, 2019]. doi:10.5455/ajpbp.20180518112401


AMA (American Medical Association) Style

Godswill Nduka Anyasor, Olamidotun Henry Ijituyi. Formulated hexane fraction of Costus afer leaves balm suppressed xylene induced topical inflammation in rat model. A J Physiol Biochem Pharmacol. 2018; 7(2): 54-60. doi:10.5455/ajpbp.20180518112401



Vancouver/ICMJE Style

Godswill Nduka Anyasor, Olamidotun Henry Ijituyi. Formulated hexane fraction of Costus afer leaves balm suppressed xylene induced topical inflammation in rat model. A J Physiol Biochem Pharmacol. (2018), [cited May 26, 2019]; 7(2): 54-60. doi:10.5455/ajpbp.20180518112401



Harvard Style

Godswill Nduka Anyasor, Olamidotun Henry Ijituyi (2018) Formulated hexane fraction of Costus afer leaves balm suppressed xylene induced topical inflammation in rat model. A J Physiol Biochem Pharmacol, 7 (2), 54-60. doi:10.5455/ajpbp.20180518112401



Turabian Style

Godswill Nduka Anyasor, Olamidotun Henry Ijituyi. 2018. Formulated hexane fraction of Costus afer leaves balm suppressed xylene induced topical inflammation in rat model. American Journal of Physiology, Biochemistry and Pharmacology, 7 (2), 54-60. doi:10.5455/ajpbp.20180518112401



Chicago Style

Godswill Nduka Anyasor, Olamidotun Henry Ijituyi. "Formulated hexane fraction of Costus afer leaves balm suppressed xylene induced topical inflammation in rat model." American Journal of Physiology, Biochemistry and Pharmacology 7 (2018), 54-60. doi:10.5455/ajpbp.20180518112401



MLA (The Modern Language Association) Style

Godswill Nduka Anyasor, Olamidotun Henry Ijituyi. "Formulated hexane fraction of Costus afer leaves balm suppressed xylene induced topical inflammation in rat model." American Journal of Physiology, Biochemistry and Pharmacology 7.2 (2018), 54-60. Print. doi:10.5455/ajpbp.20180518112401



APA (American Psychological Association) Style

Godswill Nduka Anyasor, Olamidotun Henry Ijituyi (2018) Formulated hexane fraction of Costus afer leaves balm suppressed xylene induced topical inflammation in rat model. American Journal of Physiology, Biochemistry and Pharmacology, 7 (2), 54-60. doi:10.5455/ajpbp.20180518112401





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