E-ISSN 2578-7322
 

Original Research 


Molecular Characterization of Alpha and Beta Thalassemia in Yemeni Sickle Cell Disease Children

Saeed Thabet Nasher, Mohammed Wael Abu Ghoush.

Abstract
There are no previous reports about the molecular defects of alpha and beta thalassemia in
Yemeni sickle cell disease (SCD) patients, except one report about alpha thalassemia in
Yemeni SCD children living in Riyadh Saudi Arabia.
The aim of this clinical cross-sectional study was directed to determine the genetic defects of ╬▒
and ╬▓- thalassemia in Yemeni sickle cell disease children and to determine their prevalence in
Taiz governorate Yemen where the prevalence of sickle-cell trait HbAS is about 8.2%. Blood
samples were collected from 145 SCD children to determine the complete blood count, sickling
tests and hemoglobin fraction analysis by high pressure liquid chromatography (HPLC) method.
Serum ferritin was determined in serum of those patients who had low mean cell volume
(MCV) and low mean cell hemoglobin (MCH) to exclude presence of iron deficiency anemia.
The DNA was analyzed in 30 patients with thalassemia indices after exclusion of iron
deficiency anemia using polymerase chain reaction (PCR) based techniques namely (The ╬▓-
Globin and ╬▒-globin test Strips assay and Gap PCR).
This study showed that in Taiz governorate the overall prevalence of SCD with thalassemia was
(27.5%) and according to DNA analysis the prevalence of ( HbSS+╬▒-thalassemia) and (HbS/╬▓0-
thalassemia) were 18.5% and 5.9%, respectively.
For first time in Taiz and Yemen the present study confirms presence of different combination
between sickle cell gene, ╬▓0- Thalassemia and ╬▒-Thalassemia genes which means presence of
these genetic diseases in Yemeni population. There were 8 patients (26.6%) with (╬▓S/ ╬▓0) -
thalassemia and 22 (73.3%) with (HbSS/ ╬▒ľthalassemia). There were three ╬▓ thalassemia
mutations detected the most common mutation was the ( IVS II-1 A>T ) type with frequency
of (75%), and the ╬▒-thalassemia deletion found in Yemeni SCD children was of right ward
deletion (-3.7 kb), and 17 patients (68%) had one gene deletion and 8 patients (32%) two gene
deletions.

Key words: Sickle cell, Yemen, HPLC, Thalassemia


 
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How to Cite this Article
Pubmed Style

Saeed Thabet Nasher, Mohammed Wael Abu Ghoush. Molecular Characterization of Alpha and Beta Thalassemia in Yemeni Sickle Cell Disease Children. A J Physiol Biochem Pharmacol. 2013; 2(1): 44-52. doi:10.5455/jib.20130105022014


Web Style

Saeed Thabet Nasher, Mohammed Wael Abu Ghoush. Molecular Characterization of Alpha and Beta Thalassemia in Yemeni Sickle Cell Disease Children. http://www.ajpbp.com/?mno=30701 [Access: November 18, 2018]. doi:10.5455/jib.20130105022014


AMA (American Medical Association) Style

Saeed Thabet Nasher, Mohammed Wael Abu Ghoush. Molecular Characterization of Alpha and Beta Thalassemia in Yemeni Sickle Cell Disease Children. A J Physiol Biochem Pharmacol. 2013; 2(1): 44-52. doi:10.5455/jib.20130105022014



Vancouver/ICMJE Style

Saeed Thabet Nasher, Mohammed Wael Abu Ghoush. Molecular Characterization of Alpha and Beta Thalassemia in Yemeni Sickle Cell Disease Children. A J Physiol Biochem Pharmacol. (2013), [cited November 18, 2018]; 2(1): 44-52. doi:10.5455/jib.20130105022014



Harvard Style

Saeed Thabet Nasher, Mohammed Wael Abu Ghoush (2013) Molecular Characterization of Alpha and Beta Thalassemia in Yemeni Sickle Cell Disease Children. A J Physiol Biochem Pharmacol, 2 (1), 44-52. doi:10.5455/jib.20130105022014



Turabian Style

Saeed Thabet Nasher, Mohammed Wael Abu Ghoush. 2013. Molecular Characterization of Alpha and Beta Thalassemia in Yemeni Sickle Cell Disease Children. American Journal of Physiology, Biochemistry and Pharmacology, 2 (1), 44-52. doi:10.5455/jib.20130105022014



Chicago Style

Saeed Thabet Nasher, Mohammed Wael Abu Ghoush. "Molecular Characterization of Alpha and Beta Thalassemia in Yemeni Sickle Cell Disease Children." American Journal of Physiology, Biochemistry and Pharmacology 2 (2013), 44-52. doi:10.5455/jib.20130105022014



MLA (The Modern Language Association) Style

Saeed Thabet Nasher, Mohammed Wael Abu Ghoush. "Molecular Characterization of Alpha and Beta Thalassemia in Yemeni Sickle Cell Disease Children." American Journal of Physiology, Biochemistry and Pharmacology 2.1 (2013), 44-52. Print. doi:10.5455/jib.20130105022014



APA (American Psychological Association) Style

Saeed Thabet Nasher, Mohammed Wael Abu Ghoush (2013) Molecular Characterization of Alpha and Beta Thalassemia in Yemeni Sickle Cell Disease Children. American Journal of Physiology, Biochemistry and Pharmacology, 2 (1), 44-52. doi:10.5455/jib.20130105022014





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