E-ISSN 2578-7322
 

Original Research 


Effective Removal of Insolubles from Brown Shrimp Hepatopancreatic Homogenate during Primary Stages of Alkaline Phosphatase Recovery

Krishna Prasad Nooralabettu.

Abstract
Effective centrifugal force and resident time for the primary clarification of the hepatopancreatic tissues of Brown shrimp (Metapenaeus monoceros) homogenate was performed on the optimum recovery of alkaline phosphatase. Relative Centrifugal Force (RCF) of 1681.1g for 5 min at 4oC successfully clarified 90.281.55% of the insolubles. The forces below this level even upto 20 min removed only up to 56.570.66% of the total solids and 7.900.19% of the total lipids from the homogenate. Force and time above RCF of 1681.1g for 5 min, specific activity was increased by 4.180.38 folds due to the loss of total protein, but upto 60.482.61% of alkaline phosphatase activity was lost. Hence, centrifugal force of 1681.1g for 5 min at 4oC is an effective method of clarification of tissue homogenenate.

Key words: Alkaline phosphatase, Shrimps, insoluble, centrifugation, clarification


 
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How to Cite this Article
Pubmed Style

Krishna Prasad Nooralabettu. Effective Removal of Insolubles from Brown Shrimp Hepatopancreatic Homogenate during Primary Stages of Alkaline Phosphatase Recovery. A J Physiol Biochem Pharmacol. 2014; 3(2): 66-70. doi:10.5455/jib.20140716041806


Web Style

Krishna Prasad Nooralabettu. Effective Removal of Insolubles from Brown Shrimp Hepatopancreatic Homogenate during Primary Stages of Alkaline Phosphatase Recovery. http://www.ajpbp.com/?mno=48272 [Access: November 18, 2018]. doi:10.5455/jib.20140716041806


AMA (American Medical Association) Style

Krishna Prasad Nooralabettu. Effective Removal of Insolubles from Brown Shrimp Hepatopancreatic Homogenate during Primary Stages of Alkaline Phosphatase Recovery. A J Physiol Biochem Pharmacol. 2014; 3(2): 66-70. doi:10.5455/jib.20140716041806



Vancouver/ICMJE Style

Krishna Prasad Nooralabettu. Effective Removal of Insolubles from Brown Shrimp Hepatopancreatic Homogenate during Primary Stages of Alkaline Phosphatase Recovery. A J Physiol Biochem Pharmacol. (2014), [cited November 18, 2018]; 3(2): 66-70. doi:10.5455/jib.20140716041806



Harvard Style

Krishna Prasad Nooralabettu (2014) Effective Removal of Insolubles from Brown Shrimp Hepatopancreatic Homogenate during Primary Stages of Alkaline Phosphatase Recovery. A J Physiol Biochem Pharmacol, 3 (2), 66-70. doi:10.5455/jib.20140716041806



Turabian Style

Krishna Prasad Nooralabettu. 2014. Effective Removal of Insolubles from Brown Shrimp Hepatopancreatic Homogenate during Primary Stages of Alkaline Phosphatase Recovery. American Journal of Physiology, Biochemistry and Pharmacology, 3 (2), 66-70. doi:10.5455/jib.20140716041806



Chicago Style

Krishna Prasad Nooralabettu. "Effective Removal of Insolubles from Brown Shrimp Hepatopancreatic Homogenate during Primary Stages of Alkaline Phosphatase Recovery." American Journal of Physiology, Biochemistry and Pharmacology 3 (2014), 66-70. doi:10.5455/jib.20140716041806



MLA (The Modern Language Association) Style

Krishna Prasad Nooralabettu. "Effective Removal of Insolubles from Brown Shrimp Hepatopancreatic Homogenate during Primary Stages of Alkaline Phosphatase Recovery." American Journal of Physiology, Biochemistry and Pharmacology 3.2 (2014), 66-70. Print. doi:10.5455/jib.20140716041806



APA (American Psychological Association) Style

Krishna Prasad Nooralabettu (2014) Effective Removal of Insolubles from Brown Shrimp Hepatopancreatic Homogenate during Primary Stages of Alkaline Phosphatase Recovery. American Journal of Physiology, Biochemistry and Pharmacology, 3 (2), 66-70. doi:10.5455/jib.20140716041806





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