The measurement of asymmetric dimethylarginine in human plasma by high performance liquid chromatography

Abstract

Yasemin Gulcan Kurt, Muzaffer Oztosun, ibrahim Aydin, Emin Ozgur Akgul, Enis Macit, Mehmet Agilli, Erdinc Cakir, Tuncer Cayci, Halil Yaman

Objective: We aimed to establish a high performance liquid chromatography (HPLC) method to measure plasma ADMA levels easily, sensitively and reliably. Methods: We used fluorescence detection, 50 mM sodium acetate buffer/ methanol/ Tetrahydrofuran as a mobile phase (A, 82:17:1; B, 22:77:1, % v, respectively), gradient method, 338 and 425 nm excitation and emission wavelengths, respectively. Samples were derivatizated with o-Phthaldialdehyde. Results: The recovery was found as 96 to 98,3 %. Intra-assay and inter-assay coefficients of variation (CV) were 2.3% and 2.7%, respectively. Conclusion: These findings suggest that our method is suitable for routine determination of ADMA more reliably.